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Sequencing file의 adaptor sequence 자르기와 base quality조절용 프로그램.
http://www.usadellab.org/cms/?page=trimmomatic
# 설치는 리눅스
wget http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic/Trimmomatic-0.36.zip --no-check-certificate
unzip Trimmomatic-0.36.zip
# 환경설정
vi /etc/bashrc
alias trimmomatic='java -jar /파일경로/trimmomatic-0.36.jar' #맨 밑에 쓰고 ESC+:wq
source /etc/bashrc # 동기화
trimmomatic # 실행여부확인
# 실행법
trimmomatic PE -phred33 cell10_1.fastq.gz cell10_2.fastq.gz -baseout cell10_adaptcut ILLUMINACLIP:/run/media/admin/f0bb15f3-2214-43d2-8f4f-895adde38d60/resource/ref_and_tools/tools/trimmomatic/Trimmomatic-0.36/adapters/TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:15 MINLEN:36 LEADING:3 TRAILING:3
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